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反转录酶iii 逆转录酶三 invitrogen正品原装 superscript® iii reverse transcriptase
货号18080-044
与superscriptii逆转录酶一样，superscriptiii的rnase h 活性较低，能获得更高产量的全长cdna。另外，它还有着其它的逆转录酶所无法提供的热稳定性；经过基因工程改造的superscriptiii具有更高的热稳定性，其半衰期在50度时达到令人难以置信的220分钟，这个特性也提高superscriptiii转录具有二级结构模板rna的能力。
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invitrogen：
产品描述
superscript® iii reverse transcriptase （rt） is a proprietary mutant of superscript® ii rt that is active at 50°c and has a half-life of 220 minutes, providing increased specificity with gene-specific primers （gsps） and the highest cdna yield of all rts. it is ideal for rt-pcr of a specific gene or generating cdna from total or poly （a） rna sample. like superscript® ii, it synthesizes a complementary dna strand from single-stranded rna, dna, or an rna:dna hybrid. superscript® iii rt is genetically engineered by the introduction of point mutations that increase half-life, reduce rnase activity, and increase thermal stability.
•thermostability– half life of 220 minutes at 50°c
•yield– reduced rnase h activity for more full-length cdna
•specificity– full activity at 50°c for increased specificity with gene specific primers
•applications– array labeling, cdna libraries, rt-pcr, primer extension, and 3´ and 5´ race
source: purified frome. coliexpressing thepolgene of m-mlv （1, 2）, mutagenized to increase thermal stability and half life and reduce rnase h activity.
performance and quality testing: sds-page purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays （figure 2） and yield and length of cdna product.
unit definition: one unit of superscript® iii rt is the amount of enzyme required to incorporate 1 nmole of deoxyribonucleotide into acid-precipitable material in 10 min. at 37°c using poly（a）•oligo（dt）12-18as a template•primer （3）.
unit reaction conditions: 50 mm tris-hcl （ph 8.3）, 40 mm kcl, 6 mm mgcl2, 1 mm dtt, 0.5 mm [3h]dttp, 0.1 mm poly（a）, 0.1 mm oligo（dt）12-18, 0.1 mg/ml bsa, and enzyme in 50 µl for 10 min. at 37°c.
for research use only. not intended for any animal or human therapeutic or diagnostic use.
